Microfluidic Thermal Cycler with Electrowetting

Davis, Quintin
Electrowetting takes advantage of a relationship between the interfacial energetic equilibrium of a conducting liquid and an electric field. Droplets can be actuated on a hydrophobic surface by subjection to controlled electric fields, giving the phenomenon a useful application to microfluidic solution of laboratory tasks. Many reactions in a molecular biology lab can be accomplished by temperature cycling, including the famous Polymerase Chain Reaction. In this project a number of surface treatments and morphologies were investigated as well as the control and switching of a sufficiently high voltage to actuate droplets in a microfluidic machine for completion of thermal cycling protocols in a biology laboratory.
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