Optimizing Micropropagation of Cold-hardy Grapevine Cultivars

Bergey, Daniel
Brock, Berva Dawn
Fisher, Lacey Lyn
Vardiman, Jeremiah
Dhekney, Sadanand
Grapevine bacterial and viral diseases are transmitted through infected propagation material. Micropropagation is utilized for production of disease-free stock vines. The goal of this study was to study proliferation and regeneration rates of cold-hardy grapevine cultivars. Shoot tips of 'Bronx Seedless' 'Himrod' and 'Interlaken' were obtained from greenhouse-grown grapevines. Explants were surface-sterilized by a brief rinse in 70% alcohol, agitated in a 25% commercial bleach solution for 3 min and then rinsed in distilled water. Explants were dissected to remove outer leaves and transferred onto C2D medium with 4.0 μM BAP. Cultures were maintained at 25°C, 16h/8h photoperiod and sub-cultured by transferring single nodes to fresh medium every 4 weeks. The number of shoots obtained from each explant was recorded after each transfer. After 12 weeks, shoots were transferred to rooting medium containing C2D medium plus 0.5 μM NAA. A high proliferation rate was observed in the grape cultivars studied. For instance, 'Himrod' cultures produced 8 shoots per explant during the first transfer, which increased to 152 following the second transfer. Rooted shoots were hardened in a growth chamber and transferred to a greenhouse. Proliferation rates of additional cultivars are being studied to optimize micropropagation protocols for producing disease-free grapevines.
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