Noyes, Isabel2024-02-122024-02-1210.15786/13701235https://wyoscholar.uwyo.edu/handle/internal/6538https://doi.org/10.15786/13701235This study investigates what structures and sequences the PopZ protein can bind to. I hypothesize that PopZ only binds to a specific sequence or sequences. The experiment was conducted using E. coli bacteria along with the plasmid GB1331, PCR template JH79, and a forward and 2 reverse PCR primers. The plasmid was grown and purified. The template was amplified through a PCR reaction. The plasmid and the PCR template were used in E.coli to study what and how the PopZ protein binds. This research is important because the PopZ protein is a very important polar organizing protein that is vital during cell division.enghttps://creativecommons.org/licenses/by/4.0/thesis