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Creation of a Recombinant Bacteriophage to Express Beta-Galactosidase

Swedberg, Catherine
Talbott, Heather
Abstract
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Human illness caused by the consumption of food contaminated with pathogenic bacteria is a worldwide problem that requires new detection methods to solve. The use of reporter bacteriophage as a device for detection of food-borne pathogens provides a promising new method to distinguish contaminated food and therefore prevent humans from infection. Creating a reporter bacteriophage requires modifying a bacteriophage to carry a reporter gene, which is produced during infection, allowing for rapid detection. A reporter gene was created that includes a bacteriophage promoter and the enzyme β-galactosidase. Additionally, the bacteriophage locus for recombination has been cloned. This study attempted to insert the promoter
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University of Wyoming Libraries