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Understanding the binding interface of a structurally disordered protein

Noyes, Isabel
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This study investigates what structures and sequences the PopZ protein can bind to. I hypothesize that PopZ only binds to a specific sequence or sequences. The experiment was conducted using E. coli bacteria along with the plasmid GB1331, PCR template JH79, and a forward and 2 reverse PCR primers. The plasmid was grown and purified. The template was amplified through a PCR reaction. The plasmid and the PCR template were used in E.coli to study what and how the PopZ protein binds. This research is important because the PopZ protein is a very important polar organizing protein that is vital during cell division.
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University of Wyoming. Libraries