Understanding the binding interface of a structurally disordered protein
thesisposted on 2020-05-14, 00:00 authored by Isabel Noyes
This study investigates what structures and sequences the PopZ protein can bind to. I hypothesize that PopZ only binds to a specific sequence or sequences. The experiment was conducted using E. coli bacteria along with the plasmid GB1331, PCR template JH79, and a forward and 2 reverse PCR primers. The plasmid was grown and purified. The template was amplified through a PCR reaction. The plasmid and the PCR template were used in E.coli to study what and how the PopZ protein binds. This research is important because the PopZ protein is a very important polar organizing protein that is vital during cell division.