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Surface-Enhanced Raman Spectroscopy: Applications and Methods

presentation
posted on 07.08.2014, 00:00 by Brandon Scott
Surface-enhanced Raman spectroscopy (SERS) enhances Raman scattering of molecules adsorbed to a rough metal surface by several orders of magnitude, making it a valuable tool for analytical chemistry. Gold nanoparticles, ranging from 30-50 nm in diameter, are easily synthesized in the laboratory and have a long shelf-life if properly stored, making them useful for a wide array of experiments. Alone, these particles have virtually no Raman signal, but when tagged with any Raman-sensitive molecule there is an enormous molecular signal enhancement. However, molecule-coated nanoparticles often precipitate out of solution, leading to inconsistencies with analysis and data reproducibility. This problem has been overcome by glass-coating tagged nanoparticles. These shelled and tagged nanoparticles remain stable for several months, and can function as Raman reporters for more complex research applications. Antibody-adsorbed Raman reporters can be used to selectively bind an antigen of interest. When used in conjunction with antibody-adsorbed buoyant, neutrally buoyant, or paramagnetic microparticles, reporter-bound antigen can be concentrated in a sample by settling, centrifugation, or magnetic pull-down methods, respectively. Immunoassays using the three different microparticles and Raman reporters were used to detect antigen presence.

History

Advisor

Schmit, Ginny Carron, Keith

ISO

eng

Language

English

Publisher

University of Wyoming. Libraries

Usage metrics

UGRD 2011

Keywords

Licence

Exports