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Creation of a Recombinant Bacteriophage to Express Beta-Galactosidase

presentation
posted on 07.08.2014, 00:00 by Catherine Swedberg, Heather Talbott
Human illness caused by the consumption of food contaminated with pathogenic bacteria is a worldwide problem that requires new detection methods to solve. The use of reporter bacteriophage as a device for detection of food-borne pathogens provides a promising new method to distinguish contaminated food and therefore prevent humans from infection. Creating a reporter bacteriophage requires modifying a bacteriophage to carry a reporter gene, which is produced during infection, allowing for rapid detection. A reporter gene was created that includes a bacteriophage promoter and the enzyme β-galactosidase. Additionally, the bacteriophage locus for recombination has been cloned. This study attempted to insert the promoter

History

Advisor

Willford, John

ISO

eng

Language

English

Publisher

University of Wyoming. Libraries

Usage metrics

UGRD 2011

Keywords

Licence

Exports